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mouse c2c12 myoblasts  (ATCC)


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    ATCC mouse c2c12 myoblasts
    Mouse C2c12 Myoblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse c2c12 myoblasts/product/ATCC
    Average 99 stars, based on 9232 article reviews
    mouse c2c12 myoblasts - by Bioz Stars, 2026-04
    99/100 stars

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    In vitro generation of αCD19 CAR-T cells via FuNV CAR and selective cytotoxicity against CD19 + B Cells. ( a ) The schematic diagram ofαCD19 CAR-T cells generation through membrane fusion–mediated delivery of CAR proteins by FuNV CAR . ( b ) Flow cytometry histogram of surface CAR expression on T cells at indicated time points following co-incubation with FuNV CAR (n = 5 individual replicates). ( c ) The schematic diagram of the in vitro cytotoxicity assay based on Hoechst 33,342 staining of target cells. ( d ) Quantification of cytotoxicity of FuNV CAR -generated αCD19 CAR-T cells against CD19+ B cells (n = 5 individual replicates). ( e ) Cytotoxicity of FuNV CAR -generated αCD19 CAR-T cells against CD19− control cell lines (NIH 3T3, C2C12, B16-F10 and CT26) (n = 5 individual replicates). ( f ) Representative flow cytometry plots and quantitative analysis of Annexin V/PI staining demonstrating apoptosis of CD19⁺ B cells after co-culture with FuNV CAR -generated αCD19 CAR-T cells at different doses. Data are shown as the means ± SD. ****P < 0.0001, ***P < 0.001, *P < 0.05, and ns indicates P > 0.05

    Journal: Biomarker Research

    Article Title: T-cell-targeted fusogenic nanovesicles generate CAR-T cells in vivo for rheumatoid arthritis therapy

    doi: 10.1186/s40364-026-00905-3

    Figure Lengend Snippet: In vitro generation of αCD19 CAR-T cells via FuNV CAR and selective cytotoxicity against CD19 + B Cells. ( a ) The schematic diagram ofαCD19 CAR-T cells generation through membrane fusion–mediated delivery of CAR proteins by FuNV CAR . ( b ) Flow cytometry histogram of surface CAR expression on T cells at indicated time points following co-incubation with FuNV CAR (n = 5 individual replicates). ( c ) The schematic diagram of the in vitro cytotoxicity assay based on Hoechst 33,342 staining of target cells. ( d ) Quantification of cytotoxicity of FuNV CAR -generated αCD19 CAR-T cells against CD19+ B cells (n = 5 individual replicates). ( e ) Cytotoxicity of FuNV CAR -generated αCD19 CAR-T cells against CD19− control cell lines (NIH 3T3, C2C12, B16-F10 and CT26) (n = 5 individual replicates). ( f ) Representative flow cytometry plots and quantitative analysis of Annexin V/PI staining demonstrating apoptosis of CD19⁺ B cells after co-culture with FuNV CAR -generated αCD19 CAR-T cells at different doses. Data are shown as the means ± SD. ****P < 0.0001, ***P < 0.001, *P < 0.05, and ns indicates P > 0.05

    Article Snippet: RAW264.7 cells, A20 mouse B lymphoma cells, Nalm6 human B cell leukemia cells, NIH 3T3 mouse fibroblasts, C2C12 mouse myoblasts, B16-F10 melanoma cells, and CT26 colon cancer cells were all obtained from the American Type Culture Collection.

    Techniques: In Vitro, Membrane, Flow Cytometry, Expressing, Incubation, Cytotoxicity Assay, Staining, Generated, Control, Co-Culture Assay